Volatile oil investigation:
Preparation by hydrodistillation: (E.P. 1984)
The GLC investigation:
Result
Con. %
Identified
compound
RT.
No
3.12
unknown
0.683
1
0.90
caprylic
4.067
2
0.026
pelargonic
4.950
3
0.028
Myristalic
12.833
4
15.923
palmitoleic acid
14.333
5
3.421
Margaric
15.483
6
32.51
linoleic acid
18.361
7
Microscopically examination:
1. Pollen grain spherical with spherical spiny exinle,
has 3 germinal furrows.
2. Fragments of petals with papillosed cells.
3. Fragment of fibrous layer of another which is thick
and lignified.
4. Fragment of crystal layers : parenchyma cells contain
Ca OX. crystals prisms.
5. Vessels: spiral reticulate, pitted thick, lignified.
6. Elongated cells
7. Fibers group: elongated, fuseforme, thick lignified.
Preiparation of fatty acids:
N. Hexane ext. was reflexes 2N KOH (alcoholic solution)
for 4 hours.
Extract with ether (3 x 300 ml)
Add to the aqueous extract, Hcl > till the PH is acedic.
Extract with either (3 x 300 ml)
Methylation by using Diazomethane.
